A Continuous Spectrophotometric Assay for NADPH-cytochrome P450 Reductase Activity Using 1,1-Diphenyl-2-Picrylhydrazyl
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چکیده
منابع مشابه
Spectrophotometric activity micro-assay for pure and recombinant cytochrome P450-type nitric oxide reductase Authors
Authors Seike Garny, Jan Verschoor, Neil Gardiner and Justin Jordaan Emerging Health Technologies, Council for Science and Industrial Research in Pretoria, South Africa; Department of Biochemistry, University of Pretoria, South Africa. Short title Nitric oxide reductase activity assay Corresponding author Seike Garny at Molecular Biomaterials, CSIR Biosciences, P.O. Box 395, Pretoria, 0091, Sou...
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Cytochrome P450 (P450) 2S1 is one of the orphan P450s without a clear physiological function. Controversy has arisen as to whether it can interact with NADPH-P450 reductase and accept electrons. The reduction of 1,4-bis{[2-(dimethylamino-N-oxide)ethyl]amino}-5,8-dihydroxyanthracene-9,10-dione (AQ4N) by P450 2S1 was confirmed, and the NADPH consumption rates were measured aerobically and anaerob...
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متن کاملShort Communication Cytochrome P450 2S1 is Reduced by NADPH-Cytochrome P450 Reductase
Cytochrome P450 (P450) 2S1 is one of the orphan P450s without a clear physiological function. Controversy has arisen as to whether it can interact with NADPH-P450 reductase and accept electrons. The reduction of 1,4-bis{[2-(dimethylamino-N-oxide)ethyl]amino}5,8-dihydroxyanthracene-9,10-dione (AQ4N) by P450 2S1 was confirmed, and the NADPH consumption rates were measured aerobically and anaerobi...
متن کاملShort Communication Cytochrome P450 2S1 is Reduced by NADPH-Cytochrome P450 Reductase
Cytochrome P450 (P450) 2S1 is one of the orphan P450s without a clear physiological function. Controversy has arisen as to whether it can interact with NADPH-P450 reductase and accept electrons. The reduction of 1,4-bis{[2-(dimethylamino-N-oxide)ethyl]amino}5,8-dihydroxyanthracene-9,10-dione (AQ4N) by P450 2S1 was confirmed, and the NADPH consumption rates were measured aerobically and anaerobi...
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ژورنال
عنوان ژورنال: BMB Reports
سال: 2004
ISSN: 1976-6696
DOI: 10.5483/bmbrep.2004.37.5.629